Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).
Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 5ug/ml
Western Blot: 0.1-0.3ug/ml
Immunocytochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Human kidney and human placenta lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.