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Clostridium difficile Toxin A (tcdA, toxA)

Clostridium difficile Toxin A (tcdA, toxA)

Cat no: C5851-95K

Supplier: United States Biological
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Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics. Toxin A (TcdA) is a large exotoxin which is an essential virulence factor for C. difficile infection. TcdA has a molecular weight of 308kD and is a glucosyltransferase, which irreversibly inactivates the Ras family of small GTPases to disrupt many important signalling pathways. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5851-95K
Hosts: Mouse
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in 0.01M PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Alternative names: C. difficile
Additional info: Recognizes Clostridium difficile toxin A. No reaction is observed with the following organisms: Clostridium difficile toxin B, tetanus toxoid, Pseudomonas aeruginosa and Yersinia enterocolitica.

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