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Clusterin, alpha (SGP-2, Sulfated Glycoprotein-2, DAG, Dimeric Acidic Glycoprotein, TRPM-2, Testosterone Repressed Prostate Message-2)

Cat no: C5870

Clusterin, alpha (SGP-2, Sulfated Glycoprotein-2, DAG, Dimeric Acidic Glycoprotein, TRPM-2, Testosterone Repressed Prostate Message-2)

Clusterin, which is also known as Sulfated Glycoprotein-2 (SGP-2), Dimeric Acidic Glycoprotein (DAG), and Testosterone Repressed Prostate Message-2 (TRPM-2), is a disulfide-linked heterodimeric sulfated glycoprotein expressed in a variety of tissues. Although its function is unknown, clusterin binds to cells, membranes, and hydrophobic proteins. It also inhibits complement activation. Induced or elevated levels of clusterin have been associated with programmed cell death caused by hormonal stimuli or traumatic insult. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 0.5-2ug/ml. Detects human clusterin a chain (40kD) in RIPA lysates from HeLa cells, human serum and MCF-7 cell lysates.\nImmunohistochemistry: 5ug/ml (Paraffin embedded human brain sections).\nOptimal dilutions to be determined by researcher.\n\nRecommended secondary antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5870

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 40%

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Wilson, M.R., et. al., Biochem. Biophys. Acta 1159: 319-326 (1992). 2. Narvaez, C.J., et. al., Endocrinology 137: 400-409 (1996).

Additional Info

Recognizes human clusterin, alpha at 40kD, and precursor protein.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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