Complement C3b, inactivated (iC3b, Complement Component 3, C3, AHUS5, ARMD9, ASP, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, CPAMD1)

Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. \n\nRecommended Dilutions:\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nKidney from post streptoccal glomerulonephritis patients\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7850-13V

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in borate buffered saline, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b

References

1. Janssena, B. et al. (2007) Molecular Immunology. 44: 3-10. 2. Bergmann-Leitner, E. et al. (2006) Clinical Immunology121: 177-185. 3. Sohn, J. H. et al. (2003) Nature Medicine 9: 206-212.

Additional Info

Recognizes 42kD human inavtivated comploement component 3b (iC3b) neoantigen present in blood serum.