Connexin 43 (Cx43) is a member of the connexin gene family and a structural component of gap junctions. Gap junction plaques consist of clusters of intercellular aqueous channels that permit bidirectional passage of ions, metabolites, and molecules of size up to 1kD between two neighboring cells and have been implicated to be important in the control of cell proliferation, differentiation, and regeneration. GJs also mediate electrical and metabolic coupling and ensure coordinated activity in organs such as the heart. Cx43 is the main constituent of the GJ in working cardiomyocytes, and has been the subject of numerous investigations in the context of adult cardiac function and maintenance of proper rhythm. It is believed to function as a tumor suppressor gene. Its overexpression in cancer cell lines, has been found to reduce growth and oncogenicity to varying degrees. It exists in a phosphorylated state in both neonatal and adult cardiomyocytes. It has been mapped to chromosome 6q21-q23.2. It is expressed in the heart and fetal cochlea. Pathological role of Cx43 has been implicated in autosomal dominant oculodentodigital dysplasia, syndactyly type III and left heart syndrome.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Positive Control:
Heart
Cellular Localization:
Cytoplasmic
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
