The connexins are a family of integral membrane proteins that oligomerize to form intercellular channels that are clustered at gap junctions. These channels are specialized sites of cell-cell contact that allow the passage of ions, intracellular metabolites and messenger molecules (with molecular weight less than 1-2 kD) from the cytoplasm of one cell to its opposing neighbors. They are found in almost all vertebrate cell types, and somewhat similar proteins have been cloned from plant species. Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43, the major protein of gap junctions in the heart, and gap junctions is thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 10-20ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
Testis, seminiferous tubules and leydig cells
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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