Cytochrome c pAb

Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3

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SPECIFICATIONS

Catalog Number

BS6440

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human Cytochrome c.

Species

Cytochrome c pAb detects endogenous levels of Cytochrome c protein.

Dilutions

WB: 1:500~1:2000\r\nIHC: 1:50~1:200

Swiss Prot

P99999

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 15,35 kDa

Alternative Names

Complex III subunit 4; Complex III subunit IV; CY1; CYC1; Cytochrome b c1 complex subunit 4; Cytochrome b-c1 complex subunit 4; Cytochrome bc1 complex subunit 4; Cytochrome c 1; Cytochrome c-1; Cytochrome c1; heme protein; mitochondrial; Ubiquinol cytochrome c reductase complex cytochrome c1 subunit; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; UQCR4;