Cytokeratins are constituent of the cytoskeleton, being found mainly in epithelial cells but also in some lymph node associated follicular dendritic cells and in certain endothelial and smooth muscle cells. They comprise the largest family among the intermediate filaments. There are at least 19 different cytokeratins comprised of two families; an acidic type (Type I, no 9-19) and a basic or neutral type (Type II, no 1-8). Most epithelial cells express at least one type I and one type II cytokeratin as their predominant intermediate filaments protein complement. Cytokeratin 18 is a 45kD member of the acidic family of cytokeratins, often expressed in combination with cytokeratin 8. Cytokeratin 8 and 18 (K8/18) phosphorylation plays a significant and site-specific role in regulating keratin filament organization, association with binding proteins, and modulation of cell cycle progression. The protein is expressed in colon, placenta, liver and very weakly in exocervix. The gene encoding the protein maps to 12q13 region of human chromosome. Mutations in this gene have been linked to cryptogenic cirrhosis.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 1:100
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Positive Control:
Skin
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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