Cytokeratin 18 (Keratin, Type I Cytoskeletal 18, Cell Proliferation-inducing Gene 46 Protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18, PIG46)

Cytokeratins are constituent of the cytoskeleton, being found mainly in epithelial cells but also in some lymph node associated follicular dendritic cells and in certain endothelial and smooth muscle cells. They comprise the largest family among the intermediate filaments. There are at least 19 different cytokeratins comprised of two families; an acidic type (Type I, no 9-19) and a basic or neutral type (Type II, no 1-8). Most epithelial cells express at least one type I and one type II cytokeratin as their predominant intermediate filaments protein complement. Cytokeratin 18 is a 45kD member of the acidic family of cytokeratins, often expressed in combination with cytokeratin 8. Cytokeratin 8 and 18 (K8/18) phosphorylation plays a significant and site-specific role in regulating keratin filament organization, association with binding proteins, and modulation of cell cycle progression. The protein is expressed in colon, placenta, liver and very weakly in exocervix. The gene encoding the protein maps to 12q13 region of human chromosome. Mutations in this gene have been linked to cryptogenic cirrhosis.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:100\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-21G

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Borg et al. Annals of the Rheumatic Diseases 53: 391, 1994. 2. Lauerova et al. Hybridoma 7: 495, 1988. 3. Bartek et al. J Pathol 164: 215, 1991. 4. Ramaekers et al. Am J Pathol 136: 641, 1990 5. Oshima et al. 33: 61, 1986. 6. Toivola et al. Hepatology 40: 459, 2004.

Additional Info

Recognizes human Cytokeratin 18.