Cytokeratin 19, Recombinant, Human

Keratins are a class of fibrous proteins or scleroproteins important both as structural proteins and as keys to the study of protein conformation. The family represents the principal constituent of epidermis, hair, nails, horny tissues, and the organic matrix of tooth enamel. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms an alpha-helix, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. Cytokeratin 19 is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, and in ovarian cysts, cystadenomas, and ovarian carcinomas. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix. Cytokeratin 19 has also been found in adenocarcinomas of the lung and in tumor cells of pulmonary metastases. It has been reported that cytokeratin 19 can be found in the ductal cells of normal pancreas and in pancreas cancers. It was found that ductal cells of normal pancreas express a number of cytokeratins including cytokeratin 19, whereas acinar cells of normal pancreas also express numerous cytokeratins but not cytokeratin 19. Thus it is determined that pancreas cancers consistently express certain cytokeratins including cytokeratin 19, as do normal ductal cells, which indicates that pancreas cancers may originate from normal ductal cells.\n\nRecombinant human Cytokeratin 19 is a single, non-glycosylated polypeptide chain having a molecular mass of 44,098 Dalton.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-28R

Size

5ug

Form

Supplied as a lyophilized powder in 30mM Tris-HCl, pH 8.0, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride. Reconstitute in sterile ddH2O, 0.1% BSA or HSA, to (same/more than) 0.1mg/ml, which can then be further diluted in assay buffer.

Purity

(same/more than) 95% by RP-HPLC and SDS-PAGE. Chromatographically purified.

References

1. Choi, J.H., et al., Ann. Allergy Asthma Immunol. 93(3): 293-298 (2004). 2. Deshpande, V., et al., Am. J. Surg. Pathol. 28(9): 1145-1153 (2004). 3. Favia, G., et al., Int. J. Cancer 111(5): 795-797 (2004). 4. Alvero, A.B., et al., Lab. Invest. 84(5): 658-661 (2004). 5. Pierga, J.Y., et al., Int. J. Biol. Markers 19(1): 23-31 (2004). 6. Fellowes, V.S., et al., Int. J. Oncol. 24(4): 861-867 (2004).