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Cytokeratin 5,6,18

Cat no: C9097-37G

Cytokeratin 5,6,18

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP \n\nFusion Partners: Spleen cells from immunized mice were fused with cells of the mouse p3-NSI-Ag4.1 myeloma cell line.\n\nRecommended Positive Control Tissue: Skin\n\nRecommended Negative Controls: Mouse IgG1 Negative Control\n\nApplications:\nImmunohistochemistry: (frozen)- 1:100, (paraffin)- 1:100\nOptimal working dilutions to be determined by researcher.\n\nImmunohistochemistry: #This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin. * In frozen sections recognizes cytokeratin 5, 6 and 18 with equal intensity, but staining of cytokeratin 18 in paraffin-embedded tissues may be variable.

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SPECIFICATIONS

Catalog Number

C9097-37G

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Tissue Culture Supernatant lyophilized with 15mM sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

P Type

Mab

Purity

Tissue culture supernatant.

Isotype

IgG1

References

1. Ghosh, A.K. et al. (1985). Detection of metastatic tumour cells in routine bone marrow smears by immuno-alkaline phosphatase labelling with monoclonal antibodies. Br. J. Haem. 61: 21-30.

Additional Info

Recognizes human cytokeratins 5, 6 and 18. The antibody has a broad reactivity with epithelial tissues ranging from simple glandular epithelial to stratified squamous epithelial. This reagent is valuable for the identification of epithelial tumors.

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