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DAP Kinase 1, Phosphorylated Ser308 (DAK1, DAPK1, Death Associated Protein Kinase 1)

DAP Kinase 1, Phosphorylated Ser308 (DAK1, DAPK1, Death Associated Protein Kinase 1)

Cat no: D1051

Supplier: United States Biological
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DAPK1 (Death-associated protein kinase 1) is a calmodulin dependent serine/threonine serine kinase which functions as a positive mediator of gamma-interferon induced programmed cell death. DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml using 293T (human embryonal kidney) cells transfected with DAPKinase expression vector. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D1051
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 50ul
Form: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by immunoaffinity chromatography.
References: Cohen O et al. DAP-kinase participates in TNF-alpha- and Fas-induced apoptosis and its function requires the death domain. J Cell Biol 146:141-8 (1999). Cohen O et al. DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity. EMBO J 16:998-1008 (1997). Deiss LP et al. Cathepsin D protease mediates programmed cell death induced by interferon-gamma, Fas/APO-1 and TNF-alpha. EMBO J 15:3861-70 (1996).
Additional info: Recognizes human DAP Kinase 1, phosphorylated Ser308.

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