DARPP32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Supplier:

United States Biological

Cat no: D1075-03A

DARPP32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

DARPP-32, a dopamine (DA) and cAMP-regulated ~32kD phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia,1 inhibits protein phosphatase I when it is phosphory on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75, the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission2. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac(R).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at -20 degrees C for short-term only. For long-term storage, store at -70 degrees C. Aliquots are stable for at least 12 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1075-03A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Svenningsson P, et al. Science 302:1412-1415, 2003.\n2. Nish A, et al. J Neurochem 81(4):832-841, 2002.

Additional Info

Reacts with human, mouse, and rat DARPP-32 proteins phosphorylated at Thr34. On Western blots, it identifies the target band at ~32 kD. Reactivity has been confirmed with rat caudate slices incubated in the presence of forskolin.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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