Daxx (BING2, DAP 6, DAP6, Death associated protein 6, EAP 1, EAP1, ETS1 associated protein 1, Fas death domain associated protein, hDaxx, MGC126245, MGC126246)

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx1,2. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2).\n\n\nApplications: \nSuitable for use in ELISA, Western Blotting, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50?. Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

D1150-01A

Size

100ug

Applications

ELISA, ICC, WB

Hosts

Mouse

Reactivities

Hum

Form

Liquid. 100ug of purified antibody in PBS, pH 7.4, containing 0.05% sodium azide.\n

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Yang X, et al., Cell 1997;89:1067-1076. 2. Kiriakidou M,et al., Cell Biol 1997;16:1289-1298. 3. Charette, S., et al., Mol. Cell Biol. 20:7602-7612, 2000 4. Charette, S., et al., J. Biol. Chem. 276:36071-36074, 2001

Additional Info

Recognizes human Daxx.