DC-SIGN, ED (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1)

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1-2ug/ml. A band at ~44kD can be detected.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman placenta lysate.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1550-05

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Geijtenbeek TB, et al. Cell 100:587-97(2000). 2. Pohlmann S, et al. J Virol. 75(10):4664-72 (2001). 3. Geijtenbeek TB, et al. . Cell. 2000;100(5):575-85. 4. Soilleux EJ, Barten R, Trowsdale J. . J Immunol. 2000;165(6):2937-42. 5. Pohlmann S, et al . Proc Natl Acad Sci USA. 2001;98(5):2670-2675. 6. Bashirova AA, at el.. J Exp Med. 2001;193(6):671-8. 7. Mitchell DA, Fadden AJ, Drickamer K. J Biol Chem. 2001 in press. 8. Mummidi S, et al. J Biol Chem. 2001.

Additional Info

Recognizes human DC-SIGN.