Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.
Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Peptide ELISA: 1:4000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
A431 and HeLa lysates
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.