DDB2 (Damage-specific DNA Binding Protein 2, FLJ34321)

DDB2 is required for expression of an ultraviolet radiation (UV)-damaged DNA-binding activity and is disrupted by mutations in the subset of xeroderma pigmentosum group E cells that lack this activity, DDB-negative XPE. DDB2 is the smaller subunit of a heterodimeric protein implicated in the etiology of xeroderma pigmentosum group E. This subunit appears to be required for DNA binding.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000-1:10,000\nWestern Blot: 1:500-1:1000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1584-80A

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.01% sodium azide.

P Type

Pab

Purity

Purified by delipidation and defibrination.

References

1. Shiyanov, P., et al., (1999), \"Cullin 4A associates with the UV-damaged DNA-binding protein DDB\", J. Biol. Chem., 274(50), 35309-35312. 2. Shiyanov, P., et al., (1999), \"The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription\", Mol. Cell Biol., (7), 4935-4943.

Additional Info

Recognizes human DDB2 at ~47.8kD.