DDX4 (Probable ATP-dependent RNA Helicase DDX4, DEAD Box Protein 4, Vasa Homolog, VASA)

DDX4 belongs to the DEAD box helicase family or DDX4/VASA subfamily. DEAD box proteins are characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif and are putative RNA helicases. This protein has a glycine-rich N-terminus with multiple repeats of an RGG motif, which is believed to function in RNA binding. They have a role in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. The members of this family are involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila. It is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. It is required during gametogenesis in the adult and in the specification of the germ cell lineage during embryogenesis. This is conserved in invertebrate and vertebrate species.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

D1660-26B

Size

100ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Can, NHP

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Abe, K. et al. Mammalian Genome. 8:622-623 (1997). 2. Castrillion, D. et al. Proc. Nat. Acad. Sci. 97:9585-9590 (2000). 3. Chu, D. et al. Nature. 443:101-105 (2006).

Additional Info

Recognizes DDX4. Species Crossreactivity: chimpanzee, canine, equine, human, monkey, mouse. Species sequence homology: opossum, rat.