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DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, HLR1, HELR, G17P1, DDX5)

Cat no: D1660-40G

DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, HLR1, HELR, G17P1, DDX5)

DDX5, otherwise known as p68 RNA helicase, a nuclear protein and member of the DEAD box protein family which exhibit RNA-dependent ATPase activity, and play important roles in RNA metabolism/ function, including pre-mRNA splicing, RNA translation, RNA synthesis and the assembly of RNP complexes. DDX5 interacts with Smad3 and p53 to modulate transcription, and acts as a transcriptional coactivator for estrogen receptor alpha (ERalpha).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHela lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1660-40G

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Iggo R, Gough A, Xu W, Lane DP, Spurr NK. Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc Natl Acad Sci USA. 1989 Aug;86(16):6211-4. PMID: 2762324.

Additional Info

Recognizes human DDX5. Species sequence homology: mouse and rat.

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