DDX58 pAb

The innate immune system senses viral infection by recognizing many different viral components and triggering specific antiviral responses. Intracellular double-stranded RNA (dsRNA) is a major sign of replication for many viruses. Retinoic acid inducible gene I (RIG-I) is a 925 amino acid, interferon-inducible cellular DExD/H box RNA helicase that activates type I interferon (IFN), an important effector of the innate immune system that is sensitive to these dsRNA viruses. dsRNA is normally present in very low quantities in cells, so when a virus is present, the elevated levels of dsRNA act as a sign telling RIG-I to activate the production of IFN. RIG-I does this by using its helicase domain to bind to viral dsRNA, thus transmitting the activation signal for IFN through IkB kinase-related kinases and inducing IFN expression. RIG-I is expressed in the cytoplasm of fibroblasts and conventional dendritic cells and can distinguish between many different RNA viruses.

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SPECIFICATIONS

Catalog Number

BS6819

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human DDX58.

Species

DDX58 pAb detects endogenous levels of DDX58 protein.

Dilutions

WB: 1:500~1:2000\nIHC: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

O95786

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 107 kDa

Alternative Names

Probable ATP-dependent RNA helicase DDX58; DEAD box protein 58; RIG-I-like receptor 1; RLR-1; RLR1; Retinoic acid-inducible gene 1 protein; RIG-1; RIG1; Retinoic acid-inducible gene I protein; RIG-I; RIGI;