Dectin-1 (C-type Lectin Domain Family 7 Member A, Dendritic Cell-associated C-type Lectin 1, DC-associated C-type Lectin 1, Beta-glucan Receptor, beta-GR, C-type Lectin Superfamily Member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082)

As a major leukocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens.\nRecent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilutions:\nFlow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (frozen): The epitope recognized is sensitive to H2O2 treatment, so quenching with 0.3% H2O2 should be performed after incubation with the primary antibody. The Epitope is also sensitive to formaldehyde fixation and tissue processing. Acetone fixation of frozen sections is recommended. \nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nRat Y3 myeloma cells with spleen cells from Fischer rats\n\nRecommended Secondary Antibodies:\nI1904-47Y: IgG, Fc (HRP) Pab Gt xRt\nI1904-47T: IgG, H&L (HRP) Pab Gt xRt\nI1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt\nI1904-47H: IgG, H&L (HRP) Pab Gt xRt\nI1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt\n\nRecommended Negative Control: \nI1904-82V4: IgG2b, Rat Negative Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

D1876-48P2

Size

100ug

Applications

FC, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Brown, G. D. et al. (2002) Dectin-1 is a major beta-glucan receptor on macrophages. J. Exp. Med. 196:407-412.2. Taylor, P. R. et al. (2002) The beta-glucan receptor, Dectin-1 is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J. Immunol. 169:3876-3882.3. Reid, D.M. et al. (2004) Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions. J. Leuk. Biol. 76:86-94.4. Underhill, D.M. et al. (2005) Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 106:2543-2550.

Additional Info

Recognizes mouse Dectin-1. Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.