Defender of Cell Death 1 (DAD1)

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyl-transferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.\n\nApplications: \nCan be used for detection of DAD1 by Western blot at 0.5 to 2ug/ml. Despite its predicted molecular weight, DAD1 migrates at ~22kD in SDS-PAGE. It is human and mouse reactive. For research use only. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1877-15

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

1. Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74. 2. Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9. 3. Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9. 4. Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9. 5. Hong NA, Flannery M, Hsieh SN, et al. Mice lacking Dad1, the defender against apoptotic death-1, express abnormal Nlinked glycoproteins and undergo increased embryonic apoptosis. Dev. Biol. 2000; 220:76-84. 6. Brewster JL, Martin SL, Toms J, et al. Deletion of DAD1 in mice induces an apoptosis -associated embryonic death. Genesis 2000; 26:271-8. 7. Tanaka K, Kondoh N, Shuda M, et al. Enhanced expression of mRNAs of antisecretory factor-1, gp96, DAD1 and CDC34 in human hepatocellular carcinomas. Biochim. Biophys. Acta. 2001; 1536:1-12.

Additional Info

Recognizes human DAD1.