Delta 4 (Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor) (Biotin)

Delta 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis. Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function. DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\nRecommended Dilutions:\nELISA (Detection): 0.5-2ug/ml used with D2728-01C as the capture antibody in a sandwich ELISA.\nOptimal dilutions to be determined by the researcher.\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D2728-01B

Size

250ug

Applications

ELISA

Hosts

Rat

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Labeled with Biotin. Reconstitute with 500ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313. 2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. 3. Walker, L. et al. (2001) Stem Cells 19:543. 4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. 5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.

Additional Info

Recognizes mouse Delta 4 (DLL4). Does not crossreact with recombinant human DLL4, recombinant mouse DLL1, Notch-1, Notch-2 or Notch-3.