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Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Cat no: D2727-70D

Supplier: United States Biological
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Delta-1 (also known as Delta-like 1, DLL1) is an 80-100kD vertebrate homolog of the Drosophila delta protein. Rat Delta-1 is a type I transmembrane protein that is 697aa in length. It contains a 520aa extracellular domain (ECD) and a 154aa cytoplasmic region. The ECD has one DSL segment (aa158-220) and eight consecutive EGF-like domains (aa225-515). Proteolytic processing of Delta-1 by ADAMs generates soluble intracellular and extracellular domains. Delta-1 binds to Notch and limits monocyte development while promoting T cell and DC development. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16000 Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse Heart and Rat Heart lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D2727-70D
Reactivities: Mouse, Rat
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by antigen immunoaffinity chromatography.
References: 1. Ivey KN, et al., MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. 2008 Mar 2 (3): 219-29. PMID: 18371447.
Additional info: Recognizes mouse DLL1 at ~75kD. Species Crossreactivity: rat. Species sequence homology: human.

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