Dendritic Cell Immunoactivating Receptor (DCAR, CLEC4B, C-type Lectin Domain Family 4 Member B)

DCAR is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4B. Two isoforms of DCAR exist, a 209 amino acid (aa) residue alpha isoform and a 176 aa form with a 33 aa deletion at the membrane proximal region of the extracellular domain. The DCAR extracellular domain contains a carbohydrate-recognition domain (CRD) that shares 91% amino acid sequence identity with the CRD of DCIR/CLEC4A. The DCAR intracellular domain is very short and lacks the ITIM motif found in DCIR. DCAR and DCIR are considered paired immunoregulatory receptors where DCAR activates through the ITAM of its associated adaptor molecule FcRg.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Dendritic Cell immuno-Activating Receptor alpha isoform (rmDCAR; aa 42-209; Accession # AAP58453).\n\nApplications: \nFlow Cytometry: This antibody was validated for flow cytometry using dendritic cells. Dilute this antibody to 25ug/ml and add 10 microL of the diluted solution to 1-2.5 x 10e5 dells in a total reaction volume not exceeding 200 microL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 microL of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse DCAR. The detection limit for rmDCAR is approximately 5 ng/well.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D2744-55

Size

100ug

Applications

ELISA, FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Detects rmDCAR in direct ELISAs.