Deoxyribonuclease II, CT, Blocking Peptide (DNase II)

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40kD proenzyme. The mature enzyme consists of two non-identical subunits, the 32 and 12kD (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues. \n\nThe corresponding polyclonal antibody D4020-15 can be used for detection of DNase II expression by Western blot at 1:500 to 1:1000 dilution. Tissue lysate of human spleen can be used as positive control and an approximately 32kD (a chain) band can be detected. It doesn

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SPECIFICATIONS

Catalog Number

D4020-15P

Size

1mg

Form

Supplied as a lyophilized powder.

Purity

Purified 60-70%

References

1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8\n\n2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6\n\n3. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14 \n\n4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9\n\n5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular cloning and characterization of human and murine DNase II. Gene 1998;215:281-9 \n