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Deoxyribonuclease II, Porcine spleen (DNase II)

Cat no: D3201

Deoxyribonuclease II, Porcine spleen (DNase II)

Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.\n\nApplications:\nDNA Sequencing (acidic pH)\nDNA Digestion (acidic pH)\n\nSource:\nPorcine pancreas\n\nForm: \nSupplied as a chromatographically purified, dialyzed, lyophilized powder.\n\nActivity:\n(same/more than) 12,000 units per vial\n\nUnit Definition:\n1 unit causes an increase in absorbance at 260 nm of 0.002 per minute per ml at 25C when acting upon highly polymerized DNA at pH 4.6.\nNote: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37 degrees C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction.\n\nAssay Method:\nBased on the Kunitz method where DNase will depolymerize DNA; 0.005 Kunitz units digests 1ug of lambda DNA in 10 minutes at 37 degrees C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50ul reaction. \n\nQuality Control:\nSDS-PAGE\n\nStorage and Stability:\nStable for 6-12 months at -20C\n\nCountry of Origin:\nUS certified starting material, collected in USDA approved facilities

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SPECIFICATIONS

Catalog Number

D3201

Size

20KU

Form

Salt-free, dialyzed, lyophilized powder

Purity

Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).

References

M. Kunitz, Science, 108: 19 (1948). U. Lindberg, Biochem, 6: 335 (1967). J. Salnikow, T. Liao, and W. Stein, J. Biol. Chem., 248: 1480 (1973)

Alternative Names

EC=3.1.22.1; 5

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Applications

ELISA, FC, IHC, WB

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Mouse

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IHC, WB

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Rabbit

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Hum

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Applications

ELISA, WB

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Rabbit

Reactivities

Hum

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ELISA

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Mouse

Reactivities

Hum, Mouse

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ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

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Applications

ELISA

Hosts

Mouse

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