Dermcidin (Preproteolysin, DCD, DSEP, AIDD)

The antibiotic peptide dermcidin (110aa) is specifically and constitutively expressed in the sweat glands. DCD is localized to the dark mucous cells of normal eccrine sweat glands. DCD is normally expressed in eccrine sweat glands and is expressed in cutaneous mixed tumours. DCD-immunopositive cells are expressed in the inner cells of the tubular and ductal structures.\nThe N-terminal aa sequence (18aa) is identical to a part of human cachexia-associated protein and of a survival-promoting peptide for neuronal cells. Besides this, the sequence is identical to the C-terminal peptide (aa62-79) of DCD. This monoclonal antibody can be used as a specific marker for eccrine glands in the human skin. This monoclonal antibody is useful as a immunohistochemical tool to find the tumour cells that differentiate towards eccrine secretory cells in cutaneous mixed tumours. Furthermore, the antibody can be used for the detection of biochemical and histochemical changes of the sweat gland in the healing process of wound.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50\nImmunohistochemistry: Paraffin\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3213-02

Size

1ml

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.02% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgM

References

1. Sagawa, K et al; Production and characterization of a monoclonal antibody for sweat-specific protein and its application for sweat identification. Int J Legal Med 2003, 117: 90. 2. Minami, Y et al; Immunohistochemical staining of cutaneous tumours with G-81, a monoclonal antibody to dermcidin. Br J Dermatol 2004, 151: 165.

Additional Info

Recognizes a fragment of human dermcidin.