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Desmin, Recombinant, Human (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Cat no: D3221-14

Desmin, Recombinant, Human (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Isoelectric Point:\npI 5.16 (calculated from sequence).\n\nApplications:\nSuitable for use as a Protein standard in 1D and 2D SDS gel electrophoresis, in Immunoassays and for Immunization. Other applications not tested.\n\nRecommended Dilutions: \nImmunization: The solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).\nOptimal dilutions to be determined by the researcher.\n\nReconstitution to Filaments:\nProtofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM sodium chloride, 2mM DTT, 10mM Tris-HCl, pH 7.4)\n\nStorage and Stability\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3221-14

Size

50ug

Applications

ELISA, WB

Reactivities

Hum

Form

Supplied as a liquid in 30mM Tris-HCI, pH 8, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride

Purity

(same/more than)95% (determined by SDS gel electrophoresis).

References

1. Hatzfeld, M. & Franke, W.W., J. Cell Biol. 101: 1826-1841 (1985). 2. Hatzfeld, M., et al., J. Mol. Biol. 197: 237-255 (1987).

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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