Desmoglein 1

Desmoglein 1 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Within the desmosome the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.\n\nApplications:\nCan be used for Western Blot, Immunoprecipitation and Immunofluorescence. Furthermore the antibody is useful for Immunohistology on frozen sections.\n\nRecommended Dilution:\nImmunofluorescence: 1:10 \nWestern Blot: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3221-82A

Size

100ug

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Wahl, J et al; Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21: 37.

Additional Info

Reacts with the 165kD desmoglein 1 protein. Does not inhibit the biological activity of desmoglein 1. It is not crossreactive with desmoglein 2 or desmoglein 3.