Desmoglein 3, Fc Chimera, Recombinant, Human (BSA Free)

Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130kD transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1-3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4-6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75kD ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).\n\nA DNA sequence encoding the extracellular domain of human Desmoglein-3 (Met 1-Arg 615; Accession # P32926) was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0.\n\nCalculated molecular mass of 89.5kD. As a result of glycosylation, the recombinant protein migrates as an ~ 105-110kD protein in SDS-PAGE under reducing conditions.\n\nActivity: Measured by the ability of the immobilized protein to support the adhesion of Caki-2 (human renal carcinoma epithelial cell line) cells.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3221-84C

Size

50ug

Form

Supplied as a lyophilized powder in 20mM MES, 0.15M sodium chloride, pH 6.5. Reconstitute with PBS to (same/more than)100ug/ml.

Purity

(same/more than) 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.\nEndotoxin: (same/less than)1 EU/1 ug.

References

1. Kottke, M.D. et al., 2006, J. Cell Sci. 119:797. 6. Amagai, M. et al., 1996, J. Invest. Dermatol. 106:351.\n2. Garrod, D.R. et al., 2002, Mol. Membr. Biol. 19:81. 7. Weiske, J. et al., 2001, J. Biol. Chem. 276:41175.\n3. Leckband, D. and A. Prakasam, 2006, Annu. Rev. Biomed. Eng. 8:259. 8. Cirillo, N. et al., 2007, Oral Dis. 13:341.\n4. Amagai, M. et al., 1991, Cell 67:869. 9. Wang, L. et al., 2007, Virchows Arch. Sept. 11 epub.\n5. Silos, S.A. et al., 1996, J. Biol. Chem. 271:17504. 10. Calkins, C.C. et al., 2006, J. Biol. Chem. 281:7623.