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Developmental Pluripotency-Associated Protein 4 (DPPA4)

Developmental Pluripotency-Associated Protein 4 (DPPA4)

Cat no: D3221-94R4

Supplier: United States Biological
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Developmental pluripotency associated 4 (DPPA4) protein is abundantly found in embryonic stem cells and in early embryos. DPPA4 protein is localized in the embryonic stem cell nucleus associated with transcriptionally active chromatin. The Dppa4 gene overexpression does not support embryonic stem cell self-renewal but partially inhibits embryonic stem cell differentiation. Suppression of DPPA4 expression by short hairpin RNA induced embryonic stem cell into a primitive ectoderm lineage. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D3221-94R4
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Masaki, H. et al. The Journal of Biol. Chem. 282:33034-33042 (2007). 2. Maldonado-Saldivia, J. et al. Stem Cells. 25:19-28 (2007). 3. Bortvin, A. et al. Development. 130:1673-1680 (2003).
Additional info: Recognizes human DPPA4. Species Crossreactivity: chinmpanzee, mouse, rat. Species sequence homology: monkey.

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