DFF45, CT (DNA Fragmentation Factor Subunit alpha, DNA Fragmentation Factor 45kD Subunit, DFF-45, Inhibitor of CAD, ICAD, DFFA, DFF1, H13)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chr omosomal DNA by activated DNase. A human 45kD DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3224-01A

Size

50ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Liu, X. et al. Cell 89, 175 (1997). 2. Enari, M. et al. Nature 391, 43 (1998). 3. Sakahira, H. et al. Nature 391, 96 (1998). 4. Liu, X. et al. Proc. Natl. Acad. Sci. USA 95, 8461 (1998). 5. Mukae, N. et al. Proc. Natl. Acad. Sci. USA 95, 9123 (1998). 6. Tang, D. et al. J. Biol. Chem. 273, 28549 (1998).

Additional Info

Recognizes human DFF45.