DNA fragmentation factor 45kD subunit (DFF45, also known as ICAD) is a 45kD member of the CIDE domain-containing family of proteins. It contains an N-terminal CIDE domain (aa17-96) plus two caspase-3 cleavage sites (aa117-118 and 224-225). DFF45 is a cytoplasmic protein that inhibits DFF40 DNAase. Normally, DFF45 and DFF40 form an enzymatically-inactive noncovalent heterodimer. Upon activation of the apoptotic cascade, DFF45 is cleaved into three fragments. These dissociate from DFF40 and induce DFF40 oligomerization and activation. Human DFF45 has two potential splice forms. One is truncated, and shows a seven aa substitution for aa262-331; a second is extended, and shows a 45aa substitution for the C-terminal five amino acids.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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