Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Peptide ELISA: 1:128,000.
Western Blot: 0.1-0.3microg/ml. In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kD and 45kD are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kD according to NP_115953.2. Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See D3224-71A-P. Antibody