DGK-(delta) (S66) pAb

Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-(alpha) influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-(beta) is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-(gamma) encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-(delta) is most abundant in skeletal muscle. DGK-E shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis. DGK-0 is most abundant in the cerebellum and hippocampus. DGK-l is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb. DGK-n is closely related to DGK-(delta). DGK-(zeta)is most abundant in brain and muscle. DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.

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SPECIFICATIONS

Catalog Number

BS2078

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 34-88 of Human DGK-(delta).

Species

DGK-(delta) (S66) pAb detects endogenous levels of DGK-(delta) protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

Q16760

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 135 kDa

Alternative Names

Diacylglycerol kinase delta; DAG kinase delta; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta; DGKD; KIAA0145; DGK(delta); DGK (delta)