DHPR, alpha 1 (Dihydropyridine Receptor)

Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. \nThe purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma. \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:10-1:2000. Staining of rabbit skeletal muscle results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines. \nWestern Blot: 1:500\nImmunoprecipitation: 1:10-1:500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D6500-01

Size

200ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, GP, Rabbit

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Moore RA et al. A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function. J Cell Biol 140 :843-51 (1998). PubMed: 9472036 \n\nGoligorsky MS et al. Branching points of renal resistance arteries are enriched in L-type calcium channels and initiate vasoconstriction. Am J Physiol 268 :F251-7 (1995). PubMed: 7864163 \n\nMorton ME et al. Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells. FASEB J 8:884-8 (1994). PubMed: 8070639 \n\nFlucher BE et al. Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro. J Cell Biol 123 :1161-74 (1993). PubMed: 8245124 \n\nFlucher BE et al. Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads. Neuron 5:339-51 (1990). PubMed: 2169270 \n\nMorton ME & Froehner SC The alpha 1 and alpha 2 polypeptides of the dihydropyridine-sensitive calcium channel differ in developmental expression and tissue distribution. Neuron 2:1499-506 (1989). PubMed: 2560646 \n\nMorton ME et al. Monoclonal antibody to the alpha 1-subunit of the dihydropyridine-binding complex inhibits calcium currents in BC3H1 myocytes. J Biol Chem 263 :613-6 (1988). PubMed: 2447079.

Additional Info

Detects rabbit 1,4-dihydropyridine (DHP) receptor alpha-1 subunit in skeletal muscle. \nSpecies Crossreactivity: Human, Rabbit, Rat, Mouse and Guinea pig.