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Dicer (Dicer1, DCR, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI and KIAA0928)

Cat no: 167583

Dicer (Dicer1, DCR, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI and KIAA0928)

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm\nand further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)\n\nApplications: \nWestern Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of Dicer in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody.\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167583

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Hutv

Additional Info

Recognizes mouse Dicer. Species Crossreactivity: Mouse, Rat, Human. Detects ~215kD.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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