Dihydro-Testosterone BioAssay(TM) ELISA Kit

5a-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity.\nThe bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione.\nThe major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. \n\nIntended Use:\nThe Dihydro- Testosterone ELISA kis is for the direct quantitative determination of Dihydrotestosterone by enzyme immunoassay in human serum. For in vitro diagnostic use only.\n\nPrinciple of the Test:\nThe principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHT in the sample. A set of standards is used to plot a standard curve from which the amount of DHT in patient samples and controls can be directly read.\n\nDetection Range: 0-2500 pg/ml\n\nSpecificity: 100%\n\nSensitivity: 6pg/ml\n\nKit Components:\nMicrotiter Plate, 1x96wells\nDihydrotestosterone-Horseradish Peroxidase (HRP) Conjugate Concentrate, 1x200ul\nCalibrator A 0pg/ml, 1x2ml\nCalibrator B 25pg/ml, 1x600ul\nCalibrator C 100pg/ml, 1x600ul\nCalibrator D 500pg/ml, 1x600ul\nCalibrator E 1000pg/ml, 1x600ul\nCalibrator F 2500pg/ml, 1x600ul\nControl, 1x600ul\nWash Buffer Concentrate, 1x50ml\nAssay Buffer, 1x15ml\nTMB Substrate, 1x16ml\nStop Solution, 1x6ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

167550

Size

96Tests

Applications

ELISA

References

1.Bassett, R.M.,Medical Laboratory Sciences, 37:31, 1980. 2.Baxendale, P.M., et al., Plasma and salivary androstenedione and dihydrotestosterone in women with hyperandrogenism. Clinical Endocrinology, 18:447, 1983. 3.Brooks, R.V., Androgens. Physiology and PathologyIn:Makin, H.L. J., ed., Biochemistry of Steroid Hormones, 2nd ed., Oxford Blackwell Scientific Publications, 565:1984. 4.Cameron, E.H.D., In proceedings of the fifth tenovous workshop, Steroid Immunoassay, ed., Cameron, E.H.D., et al., Alpha Omega Publishing Cordiff, 1975. 5.Dunn, J.F., et al, Transport of Steroid Hormones: Binding of 21 endogenous steroids to both SHBG and CBG in human plasma. J. Clin. Endocr. Metab. 53:58, 1981. 6.Hammond, G.L., et al, The simultaneous radioimmunoassay of seven steroids in human spermatic and peripheral venous blood. J. Clin. Endocr. Metab. 45:16, 1977.7.Ito, T., et al, Dihydrotestosterone in human peripheral plasma. J. Clin. Endocr. 31:362, 1970. 8. Mean, F., et al, Study of the binding of dihydrotestosterone, testosterone and oestradiol with sex hormone binding globulin. Clinica Cemica Alta 80:171, 1977. 9.Mooradian, A.D., et al, The biological actions of androgens. Endocr. Rev. 8:1-28, 1987. 10. Pazzagli, M., et al, Radioimmunoassay of plasma dihydrotestosterone in normal and hypogonadal men.Clin. Endocr. 82:380, 1976. 11. Wakelin, K., et al, Relationship of 5bdihydrotestosterone and 5adihydrotestosterone to testosterone inhealth and disease. J. Endocrinol. 87:450, 1980. 12. Wang, C., et al, Solitary androgens in hirsutism: Are they of use in routine evaluation. Ann. Clin.Biochem.23: 590, 1986. 13. Kricka, L.J., Human anti-animal antibody interferences in immunological assays. Clin. Chemistry 45:7, 1999. 14. Check, J.H., et al, Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest 40:139-140, 1995.