DINE (Damage-induced Neuronal Endopeptidase, ECEL1)

DINE (damage-induced neuronal endopeptidase) or Endothelin converting enzyme-like 1 (ECEL1) or X-converting enzyme (Xce) is a 95 Kda (mouse/rat/human 775 aa, chromosome 2q36-q37), type II integral membrane metalloprotease containing a conserved zinc-binding motif and an ENXADX consensus sequence, consistent with gluzinitin. The aa sequence of DINE is 36 and 32 % identical to ECE-1 and NEP, respectively. But, DINE is devoid of enzyme activity like ECE. Unlike NEP, DINE has no proteolytic activity to Ab. However, the enzyme can hydrolyze synthetic NEP substrates and thiorphan, EDTA and phosphoramidon inhibit its activity. DINE also inhibits C2-ceramide induced apoptosis in COS-7 cells. Although, the endogenous substrate for DINE is yet to be identified, its proteolytic activity activates, at least in part, free radical scavenging in damaged neurons. The DINE expression is restricted to brain tissue with predominant expression in hypothalamus, large cholinergic cells in striatum and low expression in virtually all regions of brain except in cerebral cortex, hippocampus and cerebellum. DINE, expression is markedly increased in response to optic, spinal sensory, cortical and thalamic nerve injury.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8055

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

(1) Schweizer A et al (1999) JBC 274, 20450-20456; Valdenair O et al (1999) Mol. Brain. Res. 64, 211-221; Valdenaire O et al (2000) Biochem. J. 346, 611-616; Kiryu-Seo S et al (2000) PNAS 97, 4345-4350

Additional Info

Recognizes rat DINE.