Dipeptidyl Peptidase 10 (DPP10)

DPP10 (dipeptidyl peptidase 10; also DPPY, DPRP3 and DPL2) is a 91-97kD member of the peptidase S9B family. It is a type II transmembrane glycoprotein principally expressed in neurons and T cells. Although it is a peptidase member, it has no catalytic activity. Instead, it assists in the membrane trafficking and functioning of Kv4 K+ channels. Human DPP10 is 796 amino acids (aa) in length. It contains a 34 aa cytoplasmic N-terminus and a 741 aa extracellular domain (ECD) (aa 56-796). Three potential isoforms exist, all involving aa substitutions limited to the first 20 amino acids. The ECD of human DPP10 shares 89% aa sequence identity with mouse DPP10 ECD.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human DPP10. The detection limit for rhDPP10 is approximately 2 ng/lane under non-reducing and reducing conditions. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human DPP10. The detection limit for rhDPP10 is approximately 0.3 ng/well.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8065-33

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by human DPP10 affinity chromatography.

Isotype

IgG

Additional Info

Selected for its ability to recognize human DPP10 in direct ELISAs and Western Blots. In these formats, this antibody shows less than 5% crossreactivity with rhDPP6 and rhDPP4.