Diphteria, Human, BioAssay(TM) ELISA Kit

For the quantitative determination of serum antibody response to diphtheria toxoid.\n\nDiphtheria is an acute communicable disease, caused by Corynebacterium diphtheriae. The signs and symptoms of infection are a pharyngeal membrane, sore throat, dysphasia, malaise, headache, and nausea. Death may result from respiratory obstruction by the membrane or myocarditis from the toxin.\n\nAlthough diphtheria is still a serious problem in many underdeveloped countries, active immunizations in many developed countries have helped to decrease the number of reported cases of diphtheria infection. Recent epidemics in eastern Europe and Russia, combined with low levels of protective diphtheria antitoxin (DAT) in adult populations, have caused concern that outbreaks of diphtheria could occur in developed countries. A study in northern Europe reported findings of 26% of the surveyed population being below the minimum protective level of 0.01 IU/ml.\n\nA number of methods are available for evaluating the DAT levels in body fluids. Passive hemagglutination (PHA) is widely used, but has been found to be often discrepant, rendering interpretation of the PHA test very risky for the individual patient. The use of enzyme-linked immunosorbent assays for determining DAT levels has been evaluated as simple to perform, economical, and precise. Thus the ELISA is a very practical method for seroepidemiological purposes.\n\nKit Components:\nD8065-49D1: Microtiter Plate (diphtheria toxoid antigens) 1x96wells\nD8065-49D2: Protein A (HRP) 1x11ml \nD8065-49D3: Standard 1, 0.08IU 1x2ml\nD8065-49D4: Standard 2, 0.04IU 1x2ml\nD8065-49D5: Standard 3, 0.02IU 1x2ml\nD8065-49D6: Standard 4, 0.01IU 1x2ml\nD8065-49D7: Tetramethylbenzidine (TMB) 1x11ml\nD8065-49D8: Wash Buffer, 20X 1x25ml\nD8065-49D9: Dilution Buffer 2x30ml \nD8065-49D10: Stop Solution, 1M H3PO4 1x11ml\n\nStorage and Stability:\nStore all components at 4 degrees C except D8065-49D9: Dilution Buffer. Store D8065-49D9 at RT. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts