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Diphtheria Toxin (Anatoxin)

Diphtheria Toxin (Anatoxin)

Cat no: D8065-48A

Supplier: United States Biological
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Diphtheria Toxin catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Recommended Pair: Capture: D8065-48B Detection: D8065-48A Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D8065-48A
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 1mg
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G chromatography. >95% pure.
Additional info: Recognizes Diphtheria toxin and anatoxin. Does not react with free A or B subunits of Diphtheria toxin.

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