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Diphtheria Toxin (Corynebacterium diphtheriae Toxoid, DT)

Diphtheria Toxin (Corynebacterium diphtheriae Toxoid, DT)

Cat no: D8065-42C

Supplier: United States Biological
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DT catalyzes the ADP-ribosylation of eukaryotic aminoacyl-transferase II (EF-2) using NAD as a substrate. This reaction forms the basis for its toxicity toward eukaryotic organisms. The toxin is secreted as a single polypeptide chain of about 63kD, it is then processed to form A and B fragments both of which appear to be required for toxicity. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D8065-42C
Hosts: Mouse
Applications: ELISA
Size: 100ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Additional info: Recognizes Diphtheria Toxin (DT), secreted by certain strains of Corynebacterium diphtheriae. Specific for the intact toxin/toxoid and does not react with free A or B subunits.

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