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DNA Ligase I pAb

DNA Ligase I pAb

Cat no: BS6652


Supplier: Bioworld Technology, Inc.
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Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
Catalogue number: BS6652
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: P18858
Dilutions: WB: 1:500~1:2000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Recombinant full length Human DNA Ligase I.
Species: DNA Ligase I pAb detects endogenous levels of DNA Ligase I protein.
Molecular weight: ~ 102 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: DNA ligase 1; DNA ligase I; DNLI1; LIG 1; lig1; Ligase I DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 1;
Additional info: For research use only, not for use in diagnostic procedure.

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