DNA Marker PhiX174, BsuRI (HaeIII), 11 Fragments (72-1353bp), BioGenomics(TM)

PhiX174 DNA was completely digested by BsuRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 11 discrete fragments (in base pairs): 1353, 1078, 872, 603, 310, 281, 271, 234, 194, 118, 72. \n\n6x Loading Dye Solution (L3350):\nSupplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60mM EDTA, 60% glycerol.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nRecommendations for Use:\nI. Loading on Agarose Gel\n1. Prepare DNA Marker before \nloading:\n1ul (0.5ug) of D3930-35 DNA Marker\n1ul of L3350 6X Loading Dye Solution\n4ul of deionized water \n2. Vortex gently just prior to use. Do not heat before loading.\n3. Apply the prepared amount (6ul) of \nthe DNA Marker on a 5mm lane \nof agarose gel.\n4. Following electrophoretic separation \non gel, visualize the DNA bands by \nethidium bromide staining.\nUse 0.1ug (0.2ul) of the DNA Marker (before dilution) per 1mm of an agarose gel lane width.\n\nII. Loading on Polyacrylamide Gel\n1. Prepare DNA Marker before \nloading:\n2ul (1ug) of D3930-35 DNA Marker\n0.5ul of L3350 6X Loading Dye Solution\n0.5ul of deionized water \n2. Vortex gently just prior to use. Do not heat before loading.\n3. Apply the prepared amount (3ul) of \nthe DNA Marker on a 5mm lane \nof polyacrylamide gel.\n4. Following electrophoretic separation \non gel, visualize the DNA bands by \nethidium bromide staining.\n\nUse 0.2ug (0.4ul) of the DNA Marker (before dilution) per 1mm of a Polyacrylamide gel lane width. 310, 281, 271 bp bands migrate anomalously.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

D3930-35

Size

50ug

Form

Supplied as a liquid in10mM Tris-HCl (pH 7.6), 1mM EDTA.

References

1. Stellwagen, N.C., Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels, Biochemistry 22: 6186-6193 (1983). 2. Lane, D., et al., Use of gel ratardation to analyze protein

Alternative Names

Supplied with 6x Loading Dye Solution