DNA Methyltransferase 1, phosphorylated (Ser714) (Dnmt 1, MCMT, M.Hsa1, DNA cytosine 5 methyltransferase 1, DNA MTase Mmu1, DNA methyltransferase M.HsaI)

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1000\nWestern Blot: 1:250\nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3931-61D

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. The Protein Data Bank, www.pdb.org. 2. Peterson, E.J., et al. (2003). Cancer Res. 63(20):6579-6582. 3. Leu, Y.W., et al. (2003). Cancer Res. 63(19):6110-6115. 4. Saito, Y., et al. (2003). Int. J. Cancer 105(4):527-532. 5. Siedlecki, P., et al. (2003). Biochem. Biophys. Res. Commun. 306(2):558-563. 6. Macaluso, M., et al. (2003). Oncogene 22(23):3511-3517.

Additional Info

Recognizes human Dnmt1, phosphorylated on Set714.