DNA pol E 2 pAb

DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol (delta) and E. DNA pol (delta) consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol (delta) can be regulated by cell cycle proteins. DNA pol E is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol (delta) and E on leading and lagging strands have not yet been elucidated.

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SPECIFICATIONS

Catalog Number

BS5700

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 144-189 of Human DNA pol E 2.

Species

DNA pol E 2 pAb detects endogenous levels of DNA pol E 2 protein.

Dilutions

WB: 1:500~1:1000

Swiss Prot

P56282

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 60 kDa

Alternative Names

DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B; POLE2; DPE2; DNA pol E2; DNA pol EB; DNA pol E B; DNA polE2; DNApolE2; DNA polEB; DNApolEB;