DNMT3L pAb (ChIP Grade)

Cat no: BS7226

Supplier: Bioworld Technology, Inc.

0 reviews | Write a Review Pencil

Description
Additional Information

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation.DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain.

Catalogue number:	BS7226
Reactivities:	Human
Hosts:	Rabbit
Applications:	Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size:	100ug/100ul
Swiss prot:	Q9UJW3
Dilutions:	WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:20~1:50 IP: 1:20~1:50 CHIP: 1:20~1:50
Form:	liquid
Storage buffer:	1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration:	1ug/ul
Antigen:	Recombinant full length Human DNMT3L.
Species:	DNMT3L pAb detects endogenous levels of DNMT3L protein.
Molecular weight:	~ 43 kDa
Purity:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp:	Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names:	DNA (cytosine-5)-methyltransferase 3-like; MGC1090; DNMT3L;
Additional info:	For research use only, not for use in diagnostic procedure.

See Full Product Listings >

DNMT3L pAb (ChIP Grade)

Cat no: BS7226

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from Bioworld Technology, Inc.




By submitting this form you agree to your details being passed to Bioworld Technology, Inc. for the purpose of generating the best quote*