Dopamine Transporter (DAT)

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9020-01B

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, NHP

Form

10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Erickson, J.D., Schafer, M.K.H., Bonner, T.I., Eiden, L.E. and Weihe, E. \"Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter,\" Proc. Natl. Acad. Sci. USA 93 (1996) 5166 - 5171.\n\n2. Giros, B., El Mestikawy, S., Godinot, N., Zheng, K., Han, H., Yang-Feng, T. and Caron, M.G. \"Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter,\" J. Pharmacol. Exptl. Ther. 56 (1991) 2139 - 2142.\n\n3. Haycock, J.W., Becker, L., Ang, L., Furukawa, Y., Hornykiewicz, O. and Kish, S.J. \"Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum,\" J. Neurochem. 87 (2003) 574 - 585. \n\n4. Wilson, J.M., Kalasinsky, K.S., Levey, A.I., Bergeron, C., Reiber, G., Anthony, R.M. Schmunk, G.A., Shannak, K., Haycock, J.W. and Kish, S.J., \"Striatal dopamine nerve terminal markers in human, chronic methamphetamine users,\" Nature Medicine 2 (1996) 699 - 703.\n

Additional Info

Specific for the ~88k DAT protein in Western blots of SDS-solubilized human and mouse striatal samples and in IHC applications with formaldehyde-fixed human and monkey (Macaque) brain sections. Species Crossreactivity: This antibody recognizes DAT in human, mouse, and monkey striatum.