Eg5 / KIF11 mAb

Eg5 (also known as Kinesin-like protein KIF11 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of Eg5 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. Eg5 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). Eg5 is also known to be a member of the thyroid receptor interacting protein (TRIP) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.

Prices direct from Bioworld Technology, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

MB0012

Size

100ug/100ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human Eg5.

Species

This antibody detects endogenous levels of KIF11 and does not cross-react with related proteins

Dilutions

WB: 1:1000\nICC: 1:200\nIP: 1:50~1:200

Swiss Prot

P52732

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

Predicted band size:120KDa\nObserved band size:130KDa\nAdditional band size:75K

Alternative Names

EG5; HKSP; KIF11; KIF-11; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor interacting protein 5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5; EG5; HKSP; KNSL1; TRIP5